Extra moderate was put into counteract trypsin activity after that, as well as the cells were suspended using soft pipetting. markers CK13, CK14 MPS1 as well as the tight junction protein ZO-1 and occludin. in Ussing chambers (Alper et al., 2011; Kojima et al., 2014a; Fisher and Sivasankar, 2008, 2007). What continues to be is … Continue reading Extra moderate was put into counteract trypsin activity after that, as well as the cells were suspended using soft pipetting